Microflora Involved in Phytoremediation of Polyaromatic Hydrocarbons - Annual Report

Period Covered by the Report: 8-1-00 to 7-31-01
Date of Report: 8-30-01
EPA Grant Number: R827015-01-0
Title: Microflora Involved in Phytoremediation of Polyaromatic Hydrocarbons
Investigators: David P. Nagle, John S. Fletcher
Institution: University of Oklahoma
EPA Project Officer: Bala Krishnan
Project Period: August 1, 2000 to July 31, 2001 (N/C Ext. August 1, 2001 to July 31, 2002)
Project Amount: $88,979
Research Category: Phytoremediation

Description:

WORK COMPLETED (June 1 to Aug. 31, 2001)

    1. The Smart Cycler was delivered and installed.

    2. The performance of the Smart Cycler has been studied with appropriate standards to establish its reliability.

    3. Two sets of primers have been secured and tested.

    4. Extraction and analysis of ribosomal DNA have been conducted on several microbial strains.

      5.  

      RESULTS

      1. Evaluation of the Smart Cycler in our laboratory has shown that it is fully capable of quantifying the level of dioxygenase enzymes present in micobial isolates and soil samples taken from contaminated field sites.

      2. Preliminary runs with primers have provided results that will be used to redesign the primers for more effective quantification of dioxygenase genes.

 

SIGNIFICANCE

Quantitative PCR determinations of ribosomal and nah genes have been made for the first time on plant rhizosphere samples.